- Hong Kong Hong Kong

Working Location
Job Description
Responsibilities
N-Techem International Co., Limited
About the role
We are seeking a detail-oriented Cell Culture & Bioprocessing Laboratory Technician to manage our stem cell bank and execute monthly production of Stem Cell Conditioned Medium. The primary responsibility involves thawing, expanding, and banking MSCs, executing serum-free conditioning cycles, and processing harvest fluids for downstream lyophilization (freeze-drying).
Key responsibilities
Perform routine aseptic culture, passaging, and expansion of human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) in multi-layer flasks/cell stacks (T-175, 5-Layer Stacks)
Establish and maintain Master and Working Cell Bank vials (MCB)
Execute monthly scale-up cultures for conditioned medium generation
Perform serum-free media exchanges, collection, and aseptic harvesting of conditioned medium
Carry out primary clarification of harvested Conditioned Medium
Freeze drying and formulated harvest batches
Perform routine cell counts, viability checks, and cell morphology evaluations
Conduct standard bioburden/sterility sampling and in-process testing
Maintain precise batch production records (BPR), cell banking inventories, and SOP documentation
About you
Bachelor's or Master's in biotechnology, Biology, Biochemistry, Bioengineering, or related life sciences field
1–2 years of hands-on experience with aseptic technique and mammalian cell culture (MSC, iPSC, or primary cell experience strongly preferred)
Fresh graduates with strong wet lab background are welcome to apply
High attention to detail, strong record-keeping skills, and the ability to work independently to meet monthly production targets
Fluent English
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